PHILADELPHIA â€” We couldnâ€™t live without our immune systems, always tuned to detect and eradicate invading pathogens and particles. But sometimes the immune response goes overboard, triggering autoimmune diseases like lupus, asthma or inflammatory bowel disease.
A new study led by University of Pennsylvania researchers has now identified a crucial signaling molecule involved in counterbalancing the immune system attack.
â€śThe immune response is like driving a car,â€ť said Christopher Hunter, professor and chair in the Department of Pathobiology in Pennâ€™s School of Veterinary Medicine. â€śYou hit the accelerator and develop this response thatâ€™s required to protect you from a pathogen, but, unless you have a brake to guide the response, then youâ€™ll just careen off the road and die because you canâ€™t control the speed of the response.â€ť
The research to characterize this immune system â€śbrakeâ€ť was led by Hunter and Aisling Oâ€™Hara Hall, a doctoral candidate in the Immunology Graduate Group. Additional Penn collaborators included scientists from the Penn Genome Frontiers Instituteâ€™s Department of Biology and the Perelman School of Medicineâ€™s Department of Medicine. Researchers from Merck Research Laboratories, the National Institute of Allergy and Infectious Disease, Harvard Medical School and Janssen Research and Development also contributed to the work, which was published in the journal Immunity.
â€śHealthy people have these cells â€” you have them, I have them â€” that are called Tregs,â€ť or regulatory T cells, Hunter said. â€śIf you donâ€™t have them you develop spontaneous inflammation and disease.â€ť
Different forms of regulatory T cells operate as the brakes on various kinds of inflammation, but, until now, scientists hadnâ€™t been certain of how these Tregs became specialized to do their particular jobs.
Hall, Hunter and colleagues decided to follow up on a molecule called IL-27. Scientists used to think IL-27 played a role in causing inflammation, but, in 2005, a team of Penn researchers, including Hunter, found the opposite; it was actually involved in suppressing inflammation. Thus, when mice that lack IL-27 are challenged with the parasite Toxoplasma gondii, they develop overwhelming inflammation.
â€śWe never worked out how it did that, but it was a paradigm change at the time,â€ť Hunter said.
In the new study, the researchers delved deeper into IL-27â€™s role. They found that exposing regulatory T cells to IL-27 promoted their ability to suppress a particular type of inflammation. The Penn-led team also demonstrated that they could rescue infected IL-27-deficient mice by giving them a transfusion of regulatory T cells. This finding suggests that IL-27 is required to produce the Treg cells that normally keep inflammatory responses in check during infection.
â€śVery surprisingly, we were able to show that the Tregs could ameliorate the pathology in this system,â€ť Hall said. â€śWe donâ€™t think this is the only mechanism by which IL-27 limits immune pathology, but it sheds light on one mechanism by which it could be functioning.â€ť
Further experiments showed that Tregs express a different suite of genes in the presence of IL-27 as compared to another molecule that has been implicated in this process, interferon gamma, or IFN-Îł. The researchersâ€™ findings indicate that the two molecules have division of labor when it comes to suppressing inflammation: IL-27 seems to be important in helping control inflammation at the site of inflammation, whereas IFN-Îł appears more significant in the peripheral tissues.
â€śAt the site of inflammation, where youâ€™re getting your pathology, thatâ€™s where IL- 27 is important,â€ť Hall said.
With a new understanding of how IL-27 may cause a class of Tregs to become specialized inflammation fighters, researchers have a new target for ameliorating the unwanted inflammation associated with all kinds of autoimmune conditions.
â€śNow we have a molecular signature that may be relevant in inflammatory bowel disease, in multiple sclerosis, in colitis and Crohnâ€™s disease, in rheumatoid arthritis, in lupus,â€ť Hunter said.
Next on tap, the team plans to study IL-27 in the context of asthma, lupus and arthritis.
In addition to Hall and Hunter, the authors included Beena John, Claudia GonzĂˇlez Lombana, Gretchen Harms Pritchard, Jonathan S. Silver, Jason S. Stumhofer, Tajie H. Harris, Elia D. Tait Wojno, Sagie Wagage and Philip Scott of Penn Vetâ€™s Department of Pathobiology; Daniel P. Beiting, David S. Roos and Sara Cheery of the Penn Genome Frontiers Institute Department of Biology; Steven Reiner, formerly of the Penn Department of Medicine; Cristina M. Tato and Daniel Cua of Merck Research Laboratories; Yasmine Belkaid, Guillaume Oldenhove, Nicolas Bouladoux and John Grainger of the National Institute of Allergy and Infectious Disease; Laurence A. Turka of Harvard Medical School; and M. Merle Elloso of Janssen Research and Development.
The study was supported by the Commonwealth of Pennsylvania and the National Institutes of Health.